Bacterial Efficacy of Ca(oH)2 Against E.faecalis compared with three Dental Lasers on Root Canal Dentin- An invitro study
Published: November 1, 2014 | DOI: https://doi.org/10.7860/JCDR/2014/.5215
Narasimha Reddy Kanumuru, Rama Subbaiah
1. Reader, Department of Conservative Dentistry and Endodontics, Sibar Dental College, Guntur, Andhra Pradesh, India.
2. Senior Lecturer, Department of Conservative Dentistry and Endodontics, Sibar Dental College, Guntur, Andhra Pradesh, India.
Correspondence
Dr. Narasimha Reddy Kanumuru,
Reader, Sibar Dental College, Guntur, Andhra Pradesh-522403, India.
Phone : 9160247894, E-mail : endonarsi@gmail.com
Aim: The aim of this study was to evaluate bactericidal effect of Ca(OH)2 compared with 810 nm diode, 980 nm diode, and Nd:YAG lasers on root canal dentin against E.faecalis.
Materials and Methods: Sixty five freshly extracted human mandibular single rooted teeth were selected for the study. The apical third of these roots was gradually enlarged until reaching the ISO 40- K file. The samples were divided into 4 groups, each containing 15 teeth and 5 teeth for control group. Group-1: 810 nm Diode; Group-2: 980 nm Diode; Group-3: Nd:YAG; Group-4: Ca(OH)2. 50µL of the E.faecalis ATCC 29212 strand was incubated in 1 mL of Brain Heart Infusion Broth (BHI) culture medium in37oC incubator for 4h. The concentration of the inoculation was then adjusted for a degree of turbidity which was adjusted to 0.5 McFarland scale. Later from the incubated broth, 10µL of E.faecalis culture was inoculated into the main canal and were sealed.
Results: The incubated plates were checked for growth and the colony was counted using colony counter and the results are interpreted. There was statistically significant difference (p<0.05) amongst the Ca(OH)2 group regarding the laser groups.
Conclusion: The teeth irradiated with the Nd:YAG laser had significantly higher bacterial reduction than all the other groups and the respective control groups.
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